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human fu cas9cherry expression plasmid  (Addgene inc)


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    Structured Review

    Addgene inc human fu cas9cherry expression plasmid
    Human Fu Cas9cherry Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fu+cas9cherry+expression+plasmid/FUCas9Cherry+(Plasmid+%2370182)/bio_rxiv__2025__04__28__650943-30-3-7
    Average 94 stars, based on 108 article reviews
    human fu cas9cherry expression plasmid - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Netrin-1 regulates colorectal cancer stem cell self-renewal via a TFF3 dependent paracrine survival mechanism
    Article Snippet: .. Linearisation of the human FU-Cas9Cherry expression plasmid (Addgene, Ref. #70182) was performed using the Esp□I (BsmBI) restriction enzyme (Thermo Fisher Scientific, Ref. #ER0451). .. Transfection was made using HEK293T cells and a mix including the packaging vectors (pMDLg/pRRE - Addgene #12251, pRSV-Rev - Addgene #12253 and pMD2.G/VSVg - Addgene #12259) and sgRNA-expressing DNA (1.4μg of the corresponding target-specific sgRNA or 1.4ug of sgScramble used as control) To edit our organoid cell lines using CRISPR, cells were first transduced with Cas9 and with sgRNA viruses.

    Plasmid Preparation:

    Article Title: Netrin-1 regulates colorectal cancer stem cell self-renewal via a TFF3 dependent paracrine survival mechanism
    Article Snippet: .. Linearisation of the human FU-Cas9Cherry expression plasmid (Addgene, Ref. #70182) was performed using the Esp□I (BsmBI) restriction enzyme (Thermo Fisher Scientific, Ref. #ER0451). .. Transfection was made using HEK293T cells and a mix including the packaging vectors (pMDLg/pRRE - Addgene #12251, pRSV-Rev - Addgene #12253 and pMD2.G/VSVg - Addgene #12259) and sgRNA-expressing DNA (1.4μg of the corresponding target-specific sgRNA or 1.4ug of sgScramble used as control) To edit our organoid cell lines using CRISPR, cells were first transduced with Cas9 and with sgRNA viruses.



    Similar Products

    human fu cas9cherry expression plasmid  (Addgene inc)
    94
    Addgene inc human fu cas9cherry expression plasmid
    Human Fu Cas9cherry Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fu+cas9cherry+expression+plasmid/FUCas9Cherry+(Plasmid+%2370182)/bio_rxiv__2025__04__28__650943-30-3-7
    Average 94 stars, based on 1 article reviews
    human fu cas9cherry expression plasmid - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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